diff --git a/src/IdentityServer/Endpoints/Results/JsonWebKeysResult.cs b/src/IdentityServer/Endpoints/Results/JsonWebKeysResult.cs
index 00d4e3464..4f2d056d6 100644
--- a/src/IdentityServer/Endpoints/Results/JsonWebKeysResult.cs
+++ b/src/IdentityServer/Endpoints/Results/JsonWebKeysResult.cs
@@ -55,6 +55,8 @@ public Task WriteHttpResponse(JsonWebKeysResult result, HttpContext context)
             context.Response.SetCache(result.MaxAge.Value, "Origin");
         }
 
-        return context.Response.WriteJsonAsync(new { keys = result.WebKeys }, "application/json; charset=UTF-8");
+        var json = ObjectSerializer.ToUnescapedString(new { keys = result.WebKeys });
+
+        return context.Response.WriteJsonAsync(json, "application/json; charset=UTF-8");
     }
 }
\ No newline at end of file
diff --git a/src/IdentityServer/Infrastructure/ObjectSerializer.cs b/src/IdentityServer/Infrastructure/ObjectSerializer.cs
index 23a343da4..e39c7487b 100644
--- a/src/IdentityServer/Infrastructure/ObjectSerializer.cs
+++ b/src/IdentityServer/Infrastructure/ObjectSerializer.cs
@@ -1,7 +1,8 @@
-// Copyright (c) Duende Software. All rights reserved.
+// Copyright (c) Duende Software. All rights reserved.
 // See LICENSE in the project root for license information.
 
 
+using System.Text.Encodings.Web;
 using System.Text.Json;
 using System.Text.Json.Serialization;
 
@@ -13,14 +14,34 @@ internal static class ObjectSerializer
     {
         DefaultIgnoreCondition = JsonIgnoreCondition.WhenWritingNull
     };
-        
+
+    private static readonly JsonSerializerOptions OptionsWithoutEscaping = new()
+    {
+        DefaultIgnoreCondition = JsonIgnoreCondition.WhenWritingNull,
+        // Use UnsafeRelaxedJsonEscaping to avoid escaping '+' as '\u002B' in base64-encoded
+        // values like x5c certificates. The '+' character is valid in JSON strings and does
+        // not need to be escaped. The default encoder escapes it for HTML safety, but our
+        // JSON responses are served with application/json content type.
+        Encoder = JavaScriptEncoder.UnsafeRelaxedJsonEscaping
+    };
+
     public static string ToString(object o)
     {
         return JsonSerializer.Serialize(o, Options);
     }
 
+    /// <summary>
+    /// Serializes an object to a JSON string using relaxed encoding that does not
+    /// escape characters like '+'. This is useful for producing JSON where
+    /// base64-encoded values (e.g., x5c certificates) should remain unescaped.
+    /// </summary>
+    public static string ToUnescapedString(object o)
+    {
+        return JsonSerializer.Serialize(o, OptionsWithoutEscaping);
+    }
+
     public static T FromString<T>(string value)
     {
         return JsonSerializer.Deserialize<T>(value, Options);
     }
-}
\ No newline at end of file
+}
diff --git a/test/IdentityServer.IntegrationTests/Endpoints/Discovery/DiscoveryEndpointTests.cs b/test/IdentityServer.IntegrationTests/Endpoints/Discovery/DiscoveryEndpointTests.cs
index 8af690a15..c2f0688e9 100644
--- a/test/IdentityServer.IntegrationTests/Endpoints/Discovery/DiscoveryEndpointTests.cs
+++ b/test/IdentityServer.IntegrationTests/Endpoints/Discovery/DiscoveryEndpointTests.cs
@@ -207,6 +207,62 @@ public async Task Jwks_with_two_key_using_different_algs_expect_different_alg_va
         jwks.Keys.Should().Contain(x => x.KeyId == rsaKey.KeyId && x.Alg == "RS256");
     }
 
+    [Fact]
+    [Trait("Category", Category)]
+    public async Task Jwks_x5c_should_not_escape_plus_character()
+    {
+        var cert = TestCert.Load();
+
+        IdentityServerPipeline pipeline = new IdentityServerPipeline();
+        pipeline.OnPostConfigureServices += services =>
+        {
+            services.AddIdentityServerBuilder()
+                .AddSigningCredential(cert);
+        };
+        pipeline.Initialize();
+
+        var result = await pipeline.BackChannelClient.GetAsync("https://server/.well-known/openid-configuration/jwks");
+        var json = await result.Content.ReadAsStringAsync();
+
+        // The x5c property contains base64-encoded certificate data which commonly has '+' characters.
+        // These should not be escaped as \u002B in the JSON response.
+        json.Should().NotContain("\\u002B");
+        json.Should().Contain("+");
+    }
+
+    [Fact]
+    [Trait("Category", Category)]
+    public async Task Jwks_x5t_should_not_escape_base64url_encoded_characters()
+    {
+        var cert = TestCert.Load();
+
+        IdentityServerPipeline pipeline = new IdentityServerPipeline();
+        pipeline.OnPostConfigureServices += services =>
+        {
+            services.AddIdentityServerBuilder()
+                .AddSigningCredential(cert);
+        };
+        pipeline.Initialize();
+
+        var result = await pipeline.BackChannelClient.GetAsync("https://server/.well-known/openid-configuration/jwks");
+        var json = await result.Content.ReadAsStringAsync();
+        var data = JsonSerializer.Deserialize<Dictionary<string, JsonElement>>(json);
+
+        var keys = data["keys"].EnumerateArray().ToList();
+        var keyWithX5t = keys.First(k => k.TryGetProperty("x5t", out _));
+        var x5t = keyWithX5t.GetProperty("x5t").GetString();
+
+        // The x5t property is a base64url-encoded SHA-1 thumbprint (per RFC 7517).
+        // Base64url encoding uses '-' and '_' instead of '+' and '/', so '+' and '/' must not appear.
+        x5t.Should().NotContain("+");
+        x5t.Should().NotContain("/");
+        x5t.Should().Contain("_"); // The cert we are using happens to contain '_' but not '-' in its thumbprint
+
+        // Verify the value matches the expected base64url-encoded thumbprint
+        var expectedThumbprint = Base64UrlEncoder.Encode(cert.GetCertHash());
+        x5t.Should().Be(expectedThumbprint);
+    }
+
     [Fact]
     [Trait("Category", Category)]
     public async Task Unicode_values_in_url_should_be_processed_correctly()
diff --git a/test/IdentityServer.UnitTests/Infrastructure/ObjectSerializerTests.cs b/test/IdentityServer.UnitTests/Infrastructure/ObjectSerializerTests.cs
index ea5051ff1..f64d58fcf 100644
--- a/test/IdentityServer.UnitTests/Infrastructure/ObjectSerializerTests.cs
+++ b/test/IdentityServer.UnitTests/Infrastructure/ObjectSerializerTests.cs
@@ -3,6 +3,7 @@
 
 
 using System;
+using System.Collections.Generic;
 using Duende.IdentityServer.Models;
 using FluentAssertions;
 using Xunit;
@@ -21,4 +22,20 @@ public void Can_be_deserialize_message()
         Action a = () => Duende.IdentityServer.ObjectSerializer.FromString<Message<ErrorMessage>>("{\"created\":0, \"data\": {\"error\": \"error\"}}");
         a.Should().NotThrow();
     }
+
+    [Fact]
+    public void Can_serialize_jwk_with_plus_character_in_x5c()
+    {
+        var jwk = new Dictionary<string, object>
+        {
+            { "kty", "RSA" },
+            { "x5c", new List<string> { "MIIBIjANBgkqhkiG9w0BAQEFAAOCAQ8AMIIBCgKCAQEA+test+value+with+plus" } }
+        };
+
+        var json = Duende.IdentityServer.ObjectSerializer.ToUnescapedString(jwk);
+
+        // The '+' character should not be escaped as \u002B
+        json.Should().NotContain("\\u002B");
+        json.Should().Contain("+");
+    }
 }