diff --git a/nixos/modules/system/boot/binfmt.nix b/nixos/modules/system/boot/binfmt.nix
index c9346c33deffc..c31245352846f 100644
--- a/nixos/modules/system/boot/binfmt.nix
+++ b/nixos/modules/system/boot/binfmt.nix
@@ -262,7 +262,9 @@ in
                 interpreter = mkDefault interpreterReg;
                 fixBinary = mkDefault useStaticEmulator;
                 wrapInterpreterInShell = mkDefault (!config.preserveArgvZero && !config.fixBinary);
-                interpreterSandboxPath = mkDefault (dirOf (dirOf config.interpreter));
+                interpreterSandboxPath = mkDefault (
+                  if config.fixBinary then null else dirOf (dirOf config.interpreter)
+                );
               }
               // (magics.${system} or (throw "Cannot create binfmt registration for system ${system}"))
             );
@@ -276,10 +278,13 @@ in
         let
           ruleFor = system: cfg.registrations.${system};
           hasWrappedRule = lib.any (system: (ruleFor system).wrapInterpreterInShell) cfg.emulatedSystems;
+          allFixBinary = lib.all (system: (ruleFor system).fixBinary) cfg.emulatedSystems;
         in
-        [ "/run/binfmt" ]
+        lib.optional (!allFixBinary) "/run/binfmt"
         ++ lib.optional hasWrappedRule "${pkgs.bash}"
-        ++ (map (system: (ruleFor system).interpreterSandboxPath) cfg.emulatedSystems);
+        ++ lib.filter (x: x != null) (
+          map (system: (ruleFor system).interpreterSandboxPath) cfg.emulatedSystems
+        );
     };
 
     environment.etc."binfmt.d/nixos.conf".source = builtins.toFile "binfmt_nixos.conf" (
diff --git a/nixos/tests/systemd-binfmt.nix b/nixos/tests/systemd-binfmt.nix
index 33d6cdf51de2f..bab073954a9a0 100644
--- a/nixos/tests/systemd-binfmt.nix
+++ b/nixos/tests/systemd-binfmt.nix
@@ -103,7 +103,12 @@ in
   chroot = makeTest {
     name = "systemd-binfmt-chroot";
     nodes.machine =
-      { pkgs, lib, ... }:
+      {
+        pkgs,
+        lib,
+        config,
+        ...
+      }:
       {
         boot.binfmt.emulatedSystems = [
           "aarch64-linux"
@@ -121,6 +126,13 @@ in
             echo 42 | chroot /tmp/chroot /yaml2json | grep 42
           '')
         ];
+
+        assertions = [
+          {
+            assertion = config.nix.settings.extra-sandbox-paths == [ ];
+            message = "Using binfmt_misc with static emulators, nix.settings.extra-sandbox-paths should be empty";
+          }
+        ];
       };
     testScript = ''
       machine.start()