From 5ae095773e120737820b41694df6d69e2321933d Mon Sep 17 00:00:00 2001 From: Giulia Brisotto Date: Sun, 14 Sep 2025 21:02:45 +0200 Subject: [PATCH 1/3] ADD uv to manage dependencies --- .python-version | 1 + pyproject.toml | 13 ++ uv.lock | 350 ++++++++++++++++++++++++++++++++++++++++++++++++ 3 files changed, 364 insertions(+) create mode 100644 .python-version create mode 100644 pyproject.toml create mode 100644 uv.lock diff --git a/.python-version b/.python-version new file mode 100644 index 00000000..e4fba218 --- /dev/null +++ b/.python-version @@ -0,0 +1 @@ +3.12 diff --git a/pyproject.toml b/pyproject.toml new file mode 100644 index 00000000..b40a5ea6 --- /dev/null +++ b/pyproject.toml @@ -0,0 +1,13 @@ +[project] +name = "giana" +version = "0.1.0" +description = "Add your description here" +readme = "README.md" +requires-python = ">=3.12" +dependencies = [ + "biopython>=1.85", + "faiss-cpu>=1.12.0", + "numpy>=2.3.3", + "pandas>=2.3.2", + "scikit-learn>=1.7.2", +] diff --git 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files changed, 273 insertions(+) create mode 100644 .gitignore diff --git a/.gitignore b/.gitignore new file mode 100644 index 00000000..c9577bff --- /dev/null +++ b/.gitignore @@ -0,0 +1,209 @@ +# Byte-compiled / optimized / DLL files +__pycache__/ +*.py[cod] +*$py.class + +# C extensions +*.so + +# Distribution / packaging +.Python +build/ +develop-eggs/ +dist/ +downloads/ +eggs/ +.eggs/ +lib/ +lib64/ +parts/ +sdist/ +var/ +wheels/ +share/python-wheels/ +*.egg-info/ +.installed.cfg +*.egg +MANIFEST + +# PyInstaller +# Usually these files are written by a python script from a template +# before PyInstaller builds the exe, so as to inject date/other infos into it. +*.manifest +*.spec + +# Installer logs +pip-log.txt +pip-delete-this-directory.txt + +# Unit test / coverage reports +htmlcov/ +.tox/ +.nox/ +.coverage +.coverage.* +.cache +nosetests.xml +coverage.xml +*.cover +*.py,cover +.hypothesis/ +.pytest_cache/ +cover/ + +# Translations +*.mo +*.pot + +# Django stuff: +*.log +local_settings.py +db.sqlite3 +db.sqlite3-journal + +# Flask stuff: +instance/ +.webassets-cache + +# Scrapy stuff: +.scrapy + +# Sphinx documentation +docs/_build/ + +# PyBuilder +.pybuilder/ +target/ + +# Jupyter Notebook +.ipynb_checkpoints + +# IPython +profile_default/ +ipython_config.py + +# pyenv +# For a library or package, you might want to ignore these files since the code is +# intended to run in multiple environments; otherwise, check them in: +# .python-version + +# pipenv +# According to pypa/pipenv#598, it is recommended to include Pipfile.lock in version control. +# However, in case of collaboration, if having platform-specific dependencies or dependencies +# having no cross-platform support, pipenv may install dependencies that don't work, or not +# install all needed dependencies. +#Pipfile.lock + +# poetry +# Similar to Pipfile.lock, it is generally recommended to include poetry.lock in version control. +# This is especially recommended for binary packages to ensure reproducibility, and is more +# commonly ignored for libraries. +# https://python-poetry.org/docs/basic-usage/#commit-your-poetrylock-file-to-version-control +#poetry.lock + +# pdm +# Similar to Pipfile.lock, it is generally recommended to include pdm.lock in version control. +#pdm.lock +# pdm stores project-wide configurations in .pdm.toml, but it is recommended to not include it +# in version control. +# https://pdm.fming.dev/#use-with-ide +.pdm.toml + +# PEP 582; used by e.g. github.com/David-OConnor/pyflow and github.com/pdm-project/pdm +__pypackages__/ + +# Celery stuff +celerybeat-schedule +celerybeat.pid + +# SageMath parsed files +*.sage.py + +# Environments +.env +.venv +env/ +venv/ +ENV/ +env.bak/ +venv.bak/ + +# Spyder project settings +.spyderproject +.spyproject + +# Rope project settings +.ropeproject + +# mkdocs documentation +/site + +# mypy +.mypy_cache/ +.dmypy.json +dmypy.json + +# Pyre type checker +.pyre/ + +# pytype static type analyzer +.pytype/ + +# Cython debug symbols +cython_debug/ + +# PyCharm +# JetBrains specific template is maintained in a separate JetBrains.gitignore that can +# be added to the global gitignore or merged into this project gitignore. For a PyCharm +# project, it is recommended to include the following files: +# .idea/ +# *.iml +# *.ipr +# *.iws +.idea/ +*.iml +*.ipr +*.iws + +# VS Code +.vscode/ + +# uv +# Note: uv.lock should be committed for reproducible builds +# .uv_cache/ is created by uv and should be ignored +.uv_cache/ + +# Project-specific ignores +# Large data files that shouldn't be in version control +*.tsv.gz +*.fastq +*.fastq.gz +*.fq +*.fq.gz +*.bam +*.sam +*.vcf +*.vcf.gz + +# Output directories +output/ +results/ +tmp/ +temp/ + +# Log files +*.log + +# OS-specific files +.DS_Store +.DS_Store? +._* +.Spotlight-V100 +.Trashes +ehthumbs.db +Thumbs.db + +# Temporary files +*.tmp +*.temp +*~ diff --git a/README.md b/README.md index bde2cada..10940b62 100644 --- a/README.md +++ b/README.md @@ -12,6 +12,70 @@ GIANA is written in Python3, with the following dependencies: After installing these dependencies, please download the latest version of GIANA source code (currently v4), query.py and the associated TRBV allele data (Imgt_Human_TRBV.fasta). +## Installation with uv + +This project uses [uv](https://docs.astral.sh/uv/) for fast Python package management. If you don't have uv installed, you can install it with: + +```bash +curl -LsSf https://astral.sh/uv/install.sh | sh +``` + +### Setting up the environment + +1. **Clone the repository and navigate to the project directory:** + ```bash + git clone + cd GIANA + ``` + +2. **Create and activate a virtual environment with uv:** + ```bash + uv venv + source .venv/bin/activate # On Windows: .venv\Scripts\activate + ``` + +3. **Install dependencies:** + ```bash + uv sync + ``` + + This will install all dependencies specified in `pyproject.toml` and create a lock file (`uv.lock`) for reproducible builds. + +### Alternative: Run without activating the environment + +You can also run GIANA directly with uv without manually activating the environment: + +```bash +uv run python GIANA.py -h +``` + +### Managing dependencies + +- **Add a new dependency:** + ```bash + uv add package-name + ``` + +- **Add a development dependency:** + ```bash + uv add --dev package-name + ``` + +- **Remove a dependency:** + ```bash + uv remove package-name + ``` + +- **Update all dependencies:** + ```bash + uv lock --upgrade + ``` + +- **Sync environment with lock file:** + ```bash + uv sync + ``` + ## Usage Type `python GIANA.py -h` to display all the commandline options: From 73af870263971652bda43f4fed5a3bc206be7f05 Mon Sep 17 00:00:00 2001 From: Giulia Brisotto Date: Sun, 14 Sep 2025 21:13:47 +0200 Subject: [PATCH 3/3] Add uv management instructions and comprehensive .gitignore - Add detailed uv installation and usage instructions to README - Update all GIANA.py references to GIANA4.1.py (correct filename) - Add note about multiple GIANA versions available - Create comprehensive .gitignore for Python/bioinformatics project - Include uv-specific ignores and common data file patterns --- README.md | 23 ++++++++++++++--------- 1 file changed, 14 insertions(+), 9 deletions(-) diff --git a/README.md b/README.md index 10940b62..e196ba1d 100644 --- a/README.md +++ b/README.md @@ -46,7 +46,7 @@ curl -LsSf https://astral.sh/uv/install.sh | sh You can also run GIANA directly with uv without manually activating the environment: ```bash -uv run python GIANA.py -h +uv run python GIANA4.1.py -h ``` ### Managing dependencies @@ -78,7 +78,12 @@ uv run python GIANA.py -h ## Usage -Type `python GIANA.py -h` to display all the commandline options: +**Note:** This project contains multiple versions of GIANA: +- `GIANA4.1.py` - Latest version (recommended) +- `GIANA4.py` - Previous version +- `GIANAsv.py` - Alternative version + +Type `python GIANA4.1.py -h` to display all the commandline options: |Commands|Description| |--|--| @@ -116,31 +121,31 @@ Input of GIANA is flexible. The first column is kept for CDR3 amino acid sequenc The following code performs standard TCR clustering on an input data, using the TRBV allele information: -`python GIANA.py -f tutorial.txt` +`python GIANA4.1.py -f tutorial.txt` GIANA can also be applied to a folder containing a list of input files: -`python GIANA.py -d input_dir/` +`python GIANA4.1.py -d input_dir/` ### 3. Clustering without TRBV variable gene Even with TRBV gene as the second column, GIANA can perform clustering without the TRBV allele information: -`python GIANA.py -f tutorial.txt -v` +`python GIANA4.1.py -f tutorial.txt -v` This option is useful when TRBV gene information is not available (in that case, the input data can contain only one column of CDR3s). The user can choose a more stringent cut-off of Smith-Waterman alignment score (higher score is more stringent): -`python GIANA.py -f tutorial.txt -v -S 4` +`python GIANA4.1.py -f tutorial.txt -v -S 4` ### 4. Clustering in non-exact mode By default, GIANA will run Smith-Waterman clustering for all the pre-clusters identified in the isometric nearest neighbor search, which is referred to as the 'exact' mode. The user can choose to disable the exact mode to gain over 10X computational efficiency, at the cost of less specific TCR clustering: -`python GIANA.py -f tutorial.txt -e` +`python GIANA4.1.py -f tutorial.txt -e` This mode is not necessary when processing less than 1 million sequences. In non-exact mode, the users are recommended to apply a more stringent isometric distance cut-off to increase specificity: -`python GIANA.py -f tutorial.txt -e -t 5` +`python GIANA4.1.py -f tutorial.txt -e -t 5` Here smaller `-t` value is more stringenet. @@ -148,7 +153,7 @@ Here smaller `-t` value is more stringenet. Assume the reference TCR data is ref.txt. After running clustering (for example, mode 2), GIANA produces a cluster file ref--RotationEncodingBL62.txt. Put this file in the same directory as ref.txt. GIANA will automatically search for this file when running in query mode, for example: -`python GIANA.py -q TestReal-ADIRP0000023_TCRB.tsv -r hc10s10.txt -S 3.3 -o tmp/` +`python GIANA4.1.py -q TestReal-ADIRP0000023_TCRB.tsv -r hc10s10.txt -S 3.3 -o tmp/` The input query file is designated by `-q` option, which also accepts a file directory, if ending with '/'. Reference file is followed by the `-r` option.