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Hi,
Does fastp support preprocessing of FASTQ files generated using PIPseq? PIPseq uses a different barcoding meithod from lets say 10x.
The R1 is 'Phaser (0-3bp)-t1(8bp)-Linker(3bp)-t2(6bp)-Linker(3bp)-t3(6bp)-Linker(5bp)-T4(8bp)-Bining Index(3bp)' while R2 is 'IMI(12bp)-'T'-TSO(adapter)-cDNA-Poly-A tail'
The cell barcode is : 't1+t2+t3+t4' and is used to assemble all the reads belonging to 1 cell.
Binning Index, 3 bp, Short indexing sequence used in pipseq ChemistryV to count molecules. Source : R1 after t4
Logic : BI sorts fragments into 64 bins. 4**3
MI: IMI from manual. Intrinsic Molecular Identifier. Similar to UMI. Source R2-->first 12bp
It would be a great addition to fastp if PIPseq data can be processed
Thank you
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